Healthy ageing of cloned sheep

Sinclair, Kevin D. and Corr, Sandra A. and Gutierrez, Carlos G. and Fisher, Patricia A. and Lee, J.-H. and Rathbone, Alexandra J. and Choi, Inchul and Campbell, Keith H.S. and Gardner, David S. (2016) Healthy ageing of cloned sheep. Nature Communications, 7 . 12359/1-12369/10. ISSN 2041-1723

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Available under Licence Creative Commons Attribution.
Download (1MB) | Preview
[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Available under Licence Creative Commons Attribution.
Download (198kB) | Preview

Abstract

The health of cloned animals generated by somatic-cell nuclear transfer (SCNT) has been of concern since its inception; however, there are no detailed assessments of late-onset, non-communicable diseases. Here we report that SCNT has no obvious detrimental longterm health effects in a cohort of 13 cloned sheep. We perform musculoskeletal assessments, metabolic tests and blood pressure measurements in 13 aged (7–9 years old) cloned sheep, including four derived from the cell line that gave rise to Dolly. We also perform radiological examinations of all main joints, including the knees, the joint most affected by osteoarthritis in Dolly, and compare all health parameters to groups of 5- and 6-year-old sheep, and published reference ranges. Despite their advanced age, these clones are euglycaemic, insulin sensitive and normotensive. Importantly, we observe no clinical signs of degenerative joint disease apart from mild, or in one case moderate, osteoarthritis in some animals. Our study is the first to assess the long-term health outcomes of SCNT in large animals.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences > Division of Animal Sciences
Identification Number: 10.1038/ncomms12359
Depositing User: Sinclair, Kevin
Date Deposited: 03 Nov 2016 08:42
Last Modified: 24 Jul 2017 15:45
URI: http://eprints.nottingham.ac.uk/id/eprint/38462

Actions (Archive Staff Only)

Edit View Edit View