Development of a DNA-based microarray for the detection of zoonotic pathogens in rodent species

Giles, Timothy and Yon, Lisa and Hannant, Duncan and Barrow, Paul and Abu-Median, Abu-Bakr (2015) Development of a DNA-based microarray for the detection of zoonotic pathogens in rodent species. Molecular and Cellular Probes, 29 (6). pp. 427-437. ISSN 1096-1194

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Available under Licence Creative Commons Attribution Non-commercial No Derivatives.
Download (263kB) | Preview

Abstract

The demand for diagnostic tools that allow simultaneous screening of samples for multiple pathogens is increasing because they overcome the limitations of other methods, which can only screen for a single or a few pathogens at a time. Microarrays offer the advantages of being capable to test a large number of samples simultaneously, screening for multiple pathogen types per sample and having comparable sensitivity to existing methods such as PCR. Array design is often considered the most important process in any microarray experiment and can be the deciding factor in the success of a study. There are currently no microarrays for simultaneous detection of rodent-borne pathogens. The aim of this report is to explicate the design, development and evaluation of a microarray platform for use as a screening tool that combines ease of use and rapid identification of a number of rodent-borne pathogens of zoonotic importance. Nucleic acid was amplified by multiplex biotinylation PCR prior to hybridisation onto microarrays. The array sensitivity was comparable to standard PCR, though less sensitive than real-time PCR. The array presented here is a prototype microarray identification system for zoonotic pathogens that can infect rodent species.

Item Type: Article
Keywords: Microarray; Development; Rodent; Zoonoses; ArrayTube; ArrayStrip
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: 10.1016/j.mcp.2015.07.005
Depositing User: Eprints, Support
Date Deposited: 02 Nov 2016 11:17
Last Modified: 13 Oct 2017 21:08
URI: http://eprints.nottingham.ac.uk/id/eprint/38439

Actions (Archive Staff Only)

Edit View Edit View