Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis

Harding, Stephen E. and Gillis, Richard B. and Adams, Gary G. (2016) Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis. Biophysical Reviews, 8 (4). pp. 299-308. ISSN 1867-2469

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Abstract

Molecular weights (molar masses), molecular weight distributions, dissociation constants and other interaction parameters are fundamental characteristics of proteins, nucleic acids, polysaccharides and glycoconjugates in solution. Sedimentation equilibrium in the analytical ultracentrifugation provides a powerful method with no supplementary immobilization, columns or membranes required. It is particularly powerful when used in conjunction with its sister technique, namely sedimentation velocity analysis. We describe key approaches now available and their application to the characterisation of antibodies polysaccharides and glycoconjugates. We indicate how major complications such as thermodynamic non-ideality can now be routinely dealt with, thanks to a great extent to the extensive contribution of Professor DonWinzor over several decades of research.

Item Type: Article
Keywords: SEDFIT-MSTAR; non-ideality; extended fujita; COVOL
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences
Identification Number: 10.1007/s12551-016-0232-8
Depositing User: Blay, James
Date Deposited: 01 Nov 2016 13:24
Last Modified: 13 Oct 2017 18:09
URI: http://eprints.nottingham.ac.uk/id/eprint/37909

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