Quiescent center initiation in the Arabidopsislateral root primordia is dependent on the SCARECROW transcription factor

Goh, Tatsuaki and Toyokura, Koichi and Wells, Darren M. and Swarup, Kamal and Yamamoto, Mayuko and Mimura, Tetsuro and Weijers, Dolf and Fukaki, Hidehiro and Laplaze, Laurent and Bennett, Malcolm J. and Guyomarc'h, Soazig (2016) Quiescent center initiation in the Arabidopsislateral root primordia is dependent on the SCARECROW transcription factor. Development, 143 (18). pp. 3363-3371. ISSN 1477-9129

[img] PDF - Repository staff only until 10 August 2017. - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (2MB)

Abstract

Lateral root (LR) formation is an important determinant of root system architecture. In Arabidopsis, LRs originate from pericycle cells, which undergo a programme of morphogenesis to generate a new LR meristem. Despite its importance for root meristem organisation, the onset of organizing center (termed quiescent center; QC) formation during LR morphogenesis remains unclear. Here, we used live 3D confocal imaging to monitor cell organization and identity acquisition during LR development. Our dynamic observations revealed an early morphogenesis phase and a late meristem formation phase as proposed in the bi-phasic growth model described by Sussex and co-workers. LR QC establishment coincided with this developmental phase transition. QC precursor cells originated from the outer layer of stage II LR primordia, within which the SCARECROW (SCR) transcription factor was specifically expressed. Disrupting SCR function abolished periclinal divisions in this LR primordia cell layer and perturbed the formation of QC precursor cells. We conclude that de novo QC establishment in LR primordia operates via SCR-mediated formative cell division and coincides with the developmental phase transition.

Item Type: Article
Keywords: Lateral root organogenesis, Quiescent center, SCARECROW, 3D live imaging, Arabidopsis thaliana
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences
Identification Number: https://doi.org/10.1242/dev.135319
Depositing User: Eprints, Support
Date Deposited: 24 Oct 2016 10:06
Last Modified: 26 Oct 2016 06:25
URI: http://eprints.nottingham.ac.uk/id/eprint/37855

Actions (Archive Staff Only)

Edit View Edit View