Real-time selective sequencing using nanopore technology

Loose, Matthew and Malla, Sunir and Stout, Michael (2016) Real-time selective sequencing using nanopore technology. Nature Methods, 13 (9). pp. 751-754. ISSN 1548-7105

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The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecules for sequencing by reversing the driving voltage across individual nanopores. To directly select molecules for sequencing, we used dynamic time warping to match reads to reference sequences. We demonstrate our open-source Read Until software in real-time selective sequencing of regions within small genomes, individual amplicon enrichment and normalization of an amplicon set.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences
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Depositing User: Loose, Matthew
Date Deposited: 19 Oct 2016 09:53
Last Modified: 04 May 2020 17:59

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