Real-time selective sequencing using nanopore technology

Loose, Matthew and Malla, Sunir and Stout, Michael (2016) Real-time selective sequencing using nanopore technology. Nature Methods, 13 (9). pp. 751-754. ISSN 1548-7105

[img]
Preview
PDF (BioRxiv Preprint) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (2MB) | Preview

Abstract

The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecules for sequencing by reversing the driving voltage across individual nanopores. To directly select molecules for sequencing, we used dynamic time warping to match reads to reference sequences. We demonstrate our open-source Read Until software in real-time selective sequencing of regions within small genomes, individual amplicon enrichment and normalization of an amplicon set.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: https://doi.org/10.1038/nmeth.3930
Depositing User: Loose, Matthew
Date Deposited: 19 Oct 2016 09:53
Last Modified: 01 Feb 2017 18:38
URI: http://eprints.nottingham.ac.uk/id/eprint/37695

Actions (Archive Staff Only)

Edit View Edit View