Metabolomics of aging assessed in individual parasitoid wasps

Kapranas, Apostolos and Snart, Charles J.P. and Williams, Huw Edward Llewelyn and Hardy, Ian C.W. and Barrett, David A. (2016) Metabolomics of aging assessed in individual parasitoid wasps. Scientific Reports, 6 . p. 34848. ISSN 2045-2322

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Abstract

Metabolomics studies of low-biomass organisms, such as small insects, have previously relied on the pooling of biological samples to overcome detection limits, particularly using NMR. We show that the differentiation of metabolite profiles of individual 1 mg parasitoid wasps of different ages is possible when using a modified sample preparation and a combination of untargeted NMR and LC-MS based metabolomics. Changes were observed between newly emerged and older wasps in glycerolipids, amino acids and circulatory sugars. This advance in chemical profiling has important implications for the study of the behaviour and ecology of parasitoids and many other species of small organisms because predictions and observations are typically made at the level of the individual. Thus, the metabolomic state of low-biomass individuals can now be related to their behaviour and ecological performance. We discuss specifically the utility of age-related metabolomic profiling but our new approach can be applied to a wide range of biological research.

Item Type: Article
Keywords: Ageing; behavioural ecology; chemical ecology; lipidomics; metabolomics
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences
University of Nottingham, UK > Faculty of Science > School of Chemistry
University of Nottingham, UK > Faculty of Science > School of Pharmacy
Identification Number: https://doi.org/10.1038/srep34848
Depositing User: Blay, James
Date Deposited: 19 Oct 2016 07:43
Last Modified: 19 Oct 2016 07:48
URI: http://eprints.nottingham.ac.uk/id/eprint/37662

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