Identification of sortase A (SrtA) substrates in Streptococcus uberis: evidence for an additional hexapeptide (LPXXXD) sorting motif

Egan, Sharon A. and Kurian, Dominic and Ward, Philip N. and Hunt, Lawrence and Leigh, James A. (2009) Identification of sortase A (SrtA) substrates in Streptococcus uberis: evidence for an additional hexapeptide (LPXXXD) sorting motif. Journal of Proteome Research, 9 (2). pp. 1088-1095. ISSN 1535-3907

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (421kB) | Preview

Abstract

Sortase (a transamidase) has been shown to be responsible for the covalent attachment of proteins to the bacterial cell wall. Anchoring is effected on secreted proteins containing a specific cell wall motif toward their C-terminus; that for sortase A (SrtA) in Gram-positive bacteria often incorporates the sequence LPXTG. Such surface proteins are often characterized as virulence determinants and play important roles during the establishment and persistence of infection. Intramammary infection with Streptococcus uberis is a common cause of bovine mastitis, which impacts on animal health and welfare and the economics of milk production. Comparison of stringently produced cell wall fractions from S. uberis and an isogenic mutant strain lacking SrtA permitted identification of 9 proteins likely to be covalently anchored at the cell surface. Analysis of these sequences implied the presence of two anchoring motifs for S. uberis, the classical LPXTG motif and an additional LPXXXD motif.

Item Type: Article
Additional Information: This document is the unedited author's version of a submitted work that was subsequently accepted for publication in the Journal of Proteome Research, copyright American Chemical Society. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/pr901025w
Keywords: Sortase A, LPXTG, Cell wall proteins, Mastitis, Streptococcus uberis
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: 10.1021/pr901025w
Depositing User: Egan, Sharon
Date Deposited: 07 Nov 2016 08:48
Last Modified: 13 Oct 2017 20:32
URI: http://eprints.nottingham.ac.uk/id/eprint/37634

Actions (Archive Staff Only)

Edit View Edit View