Systems analysis of auxin transport in the Arabidopsis root apex

Band, Leah R. and Wells, Darren M. and Fozard, John A. and Ghetiu, Teodor and French, Andrew P. and Pound, Michael P. and Wilson, Michael H. and Yu, Lei and Li, Wenda and Hijazi, Hussein I. and Oh, Jaesung and Pearce, Simon P. and Perez-Amador, Miguel A. and Yun, Jeonga and Kramer, Eric and Alonso, Jose M. and Godin, Christophe and Vernoux, Teva and Hodgman, T. C. and Pridmore, Tony P. and Swarup, Ranjan and King, John R. and Bennett, Malcolm J. (2014) Systems analysis of auxin transport in the Arabidopsis root apex. Plant Cell, 26 (3). pp. 862-875. ISSN 1040-4651

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Abstract

Auxin is a key regulator of plant growth and development. Within the root tip, auxin distribution plays a crucial role specifying developmental zones and coordinating tropic responses. Determining how the organ-scale auxin pattern is regulated at the cellular scale is essential to understanding how these processes are controlled. In this study, we developed an auxin transport model based on actual root cell geometries and carrier subcellular localizations. We tested model predictions using the DII-VENUS auxin sensor in conjunction with state-of-the-art segmentation tools. Our study revealed that auxin efflux carriers alone cannot create the pattern of auxin distribution at the root tip and that AUX1/LAX influx carriers are also required. We observed that AUX1 in lateral root cap (LRC) and elongating epidermal cells greatly enhance auxin’s shootward flux, with this flux being predominantly through the LRC, entering the epidermal cells only as they enter the elongation zone. We conclude that the nonpolar AUX1/LAX influx carriers control which tissues have high auxin levels, whereas the polar PIN carriers control the direction of auxin transport within these tissues.

Item Type: Article
Schools/Departments: University of Nottingham UK Campus > Faculty of Science > School of Biosciences
Identification Number: https://doi.org/10.1105/tpc.113.119495
Depositing User: Eprints, Support
Date Deposited: 18 Oct 2016 09:02
Last Modified: 20 Oct 2016 04:42
URI: http://eprints.nottingham.ac.uk/id/eprint/37628

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