Sequence and partial functional analysis of canine Bcl-2 family proteins

de Brot, Simone and Schade, Benjamin and Croci, Martina and Dettwiler, Martina and Guscetti, Franco (2016) Sequence and partial functional analysis of canine Bcl-2 family proteins. Research in Veterinary Science, 104 . pp. 126-135. ISSN 1532-2661

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Abstract

Dogs present with spontaneous neoplasms biologically similar to human cancers. Apoptotic pathways are deregulated during cancer genesis and progression and are important for therapy. We have assessed the degree of conservation of a set of canine Bcl-2 family members with the human and murine orthologs. To this end, seven complete canine open reading frames were cloned in this family, four of which are novel for the dog, their sequences were analyzed, and their functional interactions were studied in yeasts. We found a high degree of overall and domain sequence homology between canine and human proteins. It was slightly higher than between murine and human proteins. Functional interactions between canine pro-apoptotic Bax and Bak and anti-apoptotic Bcl-xL, Bcl-w, and Mcl-1 were recapitulated in yeasts. Our data provide support for the notion that systems based on canine-derived proteins might faithfully reproduce Bcl-2 family member interactions known from other species and establish the yeast as a useful tool for functional studies with canine proteins.

Item Type: Article
Keywords: Bcl-2 family; Canine; Cloning; Comparative sequence analysis; Functional yeast study
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences
University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: 10.1016/j.rvsc.2015.12.001
Depositing User: de Brot, Dr Simone
Date Deposited: 31 Oct 2016 13:05
Last Modified: 13 Oct 2017 19:10
URI: http://eprints.nottingham.ac.uk/id/eprint/37341

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