Actin depolymerization is associated with meiotic acceleration in cycloheximide-treated ovine oocytes

German, Sergio D. and Lee, Joon-Hee and Campbell, Keith H. and Sweetman, Dylan and Alberio, Ramiro (2015) Actin depolymerization is associated with meiotic acceleration in cycloheximide-treated ovine oocytes. Biology of Reproduction, 92 (4). 103-1-103-9. ISSN 1529-7268

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Abstract

Oocytes treated with the protein synthesis inhibitor cycloheximide (CHX) arrest at the germinal vesicle (GV) stage and undergo accelerated GV breakdown (GVBD) after CHX is removed. However, little is known about the underlying mechanism of accelerated meiotic maturation. Here, we investigated this mechanism and found that oocytes released from CHX arrest have higher amounts of cyclin B1 (CCNB1) and phosphorylated mitogen-activated protein kinase (pMAPK) proteins. Increased levels of these factors were not associated with mRNA polyadenylation or increased transcription rates of CCNB1 and MOS (Moloney murine sarcoma viral oncogene homolog) during CHX arrest. We found that treatment of CHX-arrested oocytes with the actin filament-stabilizing agent Jasplakinolide (Jasp) delayed GVBD following release from CHX arrest and that this was correlated with reduced maturation-promoting factor (MPF) activity. These results suggest that CCNB1 mRNAs released from actin filaments during CHX arrest increase CCNB1 transcripts available for translation after release from CHX arrest, leading to the precocious activation of MPF and accelerated meiotic progression.

Item Type: Article
Keywords: actin depolymerization, cyclin B1, cycloheximide, cytoskeleton, GVBD, IVM, meiotic arrest, meiotic maturation, oocyte maturation, polyadenylation, protein kinases, RNA granules
Schools/Departments: University of Nottingham UK Campus > Faculty of Science > School of Biosciences
University of Nottingham UK Campus > Faculty of Science > School of Biosciences > Division of Animal Sciences
Identification Number: https://doi.org/10.1095/biolreprod.114.122341
Depositing User: Eprints, Support
Date Deposited: 22 Sep 2016 13:37
Last Modified: 23 Sep 2016 16:15
URI: http://eprints.nottingham.ac.uk/id/eprint/37068

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