The application of protein microarray assays in psychoneuroimmunology

Ayling, K. and Bowden, T. and Tighe, Patrick J. and Todd, Ian and Dilnot, Elizabeth M. and Hegm, O.H. and Faircolugh, L. and Vedhara, Kavita (2016) The application of protein microarray assays in psychoneuroimmunology. Brain, Behavior and Immunity . ISSN 1090-2139 (In Press)

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Protein microarrays are miniaturized multiplex assays that exhibit many advantages over the commonly used enzyme-linked immunosorbent assay (ELISA). This article aims to introduce protein microarrays to readers of Brain, Behavior, and Immunity and demonstrate its utility and validity for use in psychoneuroimmunological research. As part of an ongoing investigation of psychological and behavioral influences on influenza vaccination responses, we optimized a novel protein microarray to quantify influenza-specific antibody levels in human sera. Reproducibility was assessed by calculating intra- and inter-assay coefficients of variance on serially diluted human IgG concentrations. A random selection of samples was analyzed by microarray and ELISA to establish validity of the assay. For IgG concentrations, intra-assay and inter-assay precision profiles demonstrated a mean coefficient of variance of 6.7% and 11.5% respectively. Significant correlations were observed between microarray and ELISA for all antigens, demonstrating the microarray is a valid alternative to ELISA. Protein microarrays are a highly robust, novel assay method that could be of significant benefit for researchers working in psychoneuroimmunology. They offer high throughput, fewer resources per analyte and can examine concurrent neuro-immune-endocrine mechanisms.

Item Type: Article
Additional Information: © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Keywords: Microarray; ELISA; Psychoneuroimmunology; Sera; Multiplex; Assay
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Medicine > Division of Primary Care
University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Life Sciences
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Depositing User: McCambridge, Mrs April
Date Deposited: 23 Sep 2016 07:20
Last Modified: 25 Sep 2016 04:16

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