A genetic assay for gene essentiality in Clostridium

Walker, David J.F. and Heap, John T. and Winzer, Klaus and Minton, Nigel P. (2016) A genetic assay for gene essentiality in Clostridium. Anaerobe . ISSN 1095-8274

[img] PDF - Repository staff only - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (1MB)

Abstract

Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a stable second copy of the gene.

Item Type: Article
Keywords: Clostridium difficile; ClosTron; merodiploid; gene essentiality; therapeutic target
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Medicine > Nottingham Digestive Diseases Centre
University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: https://doi.org/10.1016/j.anaerobe.2016.07.007
Depositing User: Eprints, Support
Date Deposited: 22 Aug 2016 07:23
Last Modified: 14 Sep 2016 13:21
URI: http://eprints.nottingham.ac.uk/id/eprint/35788

Actions (Archive Staff Only)

Edit View Edit View