A genetic assay for gene essentiality in Clostridium

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Walker, David J.F., Heap, John T., Winzer, Klaus and Minton, Nigel P. (2016) A genetic assay for gene essentiality in Clostridium. Anaerobe, 42 . pp. 40-43. ISSN 1095-8274

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Official URL: http://www.sciencedirect.com/science/article/pii/S1075996416300919

Abstract

Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a stable second copy of the gene.

Item Type:	Article
RIS ID:	https://nottingham-repository.worktribe.com/output/831627
Keywords:	Clostridium difficile; ClosTron; merodiploid; gene essentiality; therapeutic target
Schools/Departments:	University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine > Nottingham Digestive Diseases Centre University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number:	https://doi.org/10.1016/j.anaerobe.2016.07.007
Depositing User:	Eprints, Support
Date Deposited:	22 Aug 2016 07:23
Last Modified:	04 May 2020 18:23
URI:	https://eprints.nottingham.ac.uk/id/eprint/35788

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