Yang, Xin, Hamner, Margaret A., Brown, Angus M., Evans, Richard D., Ye, Zu-Cheng, Chen, Shengdi and Ransom, Bruce R.
(2014)
Novel hypoglycemic injury mechanism: N-methyl-D-aspartate receptor-mediated white matter damage.
Annals of Neurology, 75
(4).
pp. 492-507.
ISSN 0364-5134
Full text not available from this repository.
Abstract
Objective: Hypoglycemia is a common adverse event and can injure central nervous system (CNS) white matter (WM). We determined if glutamate receptors were involved in hypoglycemic WM injury.
Methods: Mouse optic nerves (MON), CNS WM tracts, were maintained at 37°C with oxygenated artificial cerebrospinal fluid (ACSF) containing 10 mM glucose. Aglycemia was produced by switching to 0 glucose ACSF. Supra-maximal compound action potentials (CAPs) were elicited using suction electrodes and axon function was quantified as the area under the CAP. Amino acid release was measured using HPLC. Extracellular [lactate] was measured using an enzyme electrode.
Results: About 50% of MON axons were injured after 60 min of aglycemia (90% after 90 min); injury was not affected by animal age. Blockade of NMDA-type glutamate receptors improved recovery after 90 min of aglycemia by 250%. Aglycemic injury was increased by reducing [Mg2+]o or increasing [glycine]o, and decreased by lowering pHo, expected results for NMDA receptor-mediated injury. Extracellular pH increased during aglycemia, due to a drop in [lactate-]o. Aglycemic injury was dramatically reduced in the absence of [Ca2+]o. Extracellular aspartate, a selective NMDA receptor agonist, increased during aglycemia.
Interpretation: Aglycemia injured WM by a unique excitotoxic mechanism involving NMDA receptors (located primarily on oligodendrocytes). During WM aglycemia, the selective NMDA agonist, aspartate, is released, probably from astrocytes. Injury is mediated by Ca2+ influx through aspartate-activated NMDA receptors made permeable by an accompanying alkaline shift in pHo caused by a fall in [lactate-]o. These insights have important clinical implications.
| Item Type: |
Article
|
| RIS ID: |
https://nottingham-repository.worktribe.com/output/727206 |
| Additional Information: |
This is the peer reviewed version of the following article: Yang, X., Hamner, M. A., Brown, A. M., Evans, R. D., Ye, Z.-C., Chen, S. and Ransom, B. R. (2014), Novel hypoglycemic injury mechanism: N-methyl-D-aspartate receptor–mediated white matter damage. Ann Neurol., 75: 492–507. doi: 10.1002/ana.24050, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/ana.24050/abstract. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Schools/Departments: |
University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences > School of Biomedical Sciences |
| Identification Number: |
https://doi.org/10.1002/ana.24050 |
| Depositing User: |
Eprints, Support
|
| Date Deposited: |
25 Jul 2016 14:02 |
| Last Modified: |
04 May 2020 16:46 |
| URI: |
https://eprints.nottingham.ac.uk/id/eprint/35413 |
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