Hydrotropism: analysis of the root response to a moisture gradient

Antoni, Regina and Dietrich, Daniela and Bennett, Malcolm J. and Rodriguez, Pedro L. (2016) Hydrotropism: analysis of the root response to a moisture gradient. Methods in Molecular Biology, 1398 . pp. 3-9. ISSN 1940-6029

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Abstract

Hydrotropism is a genuine response of roots to a moisture gradient to avoid drought. An experimental system for the induction of hydrotropic root response in petri dishes was designed by pioneering groups in the field. This system uses split agar plates containing an osmolyte only in a region of the plate in order to generate a water potential gradient. Arabidopsis seedlings are placed on the MS agar plate so that their root tips are near the junction between plain MS medium and the region supplemented with the osmolyte. This elicits a hydrotropic response in Arabidopsis roots that can be measured as the root curvature angle.

Item Type: Article
Additional Information: The final publication is available at Springer via http://dx.doi.org/10.1007/978-1-4939-3356-3_1
Keywords: Hydrotropism, Water potential gradient, Root curvature angle, Moisture gradient, Root growth, Sorbitol, ABA, Arabidopsis
Schools/Departments: University of Nottingham UK Campus > Faculty of Science > School of Biosciences
Identification Number: https://doi.org/10.1007/978-1-4939-3356-3_1
Depositing User: Hatton, Mrs Kirsty
Date Deposited: 25 Jul 2016 12:41
Last Modified: 24 Sep 2016 21:45
URI: http://eprints.nottingham.ac.uk/id/eprint/35401

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