Immediate early protein of equid herpesvirus type 1 as a target for cytotoxic T-lymphocytes in the thoroughbred horse

Kydd, Julia H. and Case, Ruth and Minke, Julius and Audonnet, Jean-Christophe and Wagner, Bettina and Antczak, Douglas F. (2014) Immediate early protein of equid herpesvirus type 1 as a target for cytotoxic T-lymphocytes in the thoroughbred horse. Journal of General Virology, 95 . pp. 1783-1789. ISSN 1465-2099

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (256kB) | Preview

Abstract

Cytotoxic T-lymphocytes (CTLs) are associated with protective immunity against disease caused by equid herpesvirus type 1 (EHV-1). However, the EHV-1 target proteins for CTLs are poorly defined. This limits the development of vaccine candidates designed to stimulate strong CTL immunity. Here, classical CTL assays using lymphocytes from horses of three defined MHC class I types that experienced natural infection with EHV-1 and a modified vaccinia virus construct containing an EHV-1 gene encoding the immediate-early (IE) protein are reported. Horses homozygous for the equine leukocyte antigen (ELA)-A2 haplotype, but not the ELA-A5 haplotype, produced MHC-restricted CTL responses against the IE protein. Previously, horses homozygous for the ELA-A3 haplotype also mounted CTL responses against the IE protein. Both haplotypes are common in major horse breeds, including the Thoroughbred. Thus, the IE protein is an attractive candidate molecule for future studies of T-cell immunity to EHV-1 in the horse.

Item Type: Article
Additional Information: This is the author's version of a work that was accepted for publication in Journal of General Virology. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of General Virology Vol. 95, pp. 1783-1789 and is available at http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.065888-0
Keywords: Equine, Herpesvirus, Thoroughbred, Cytotoxic T lymphocytes
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: https://doi.org/10.1099/vir.0.065888-0
Depositing User: Kydd, Julia
Date Deposited: 21 Jul 2016 12:19
Last Modified: 16 Sep 2016 01:39
URI: http://eprints.nottingham.ac.uk/id/eprint/35230

Actions (Archive Staff Only)

Edit View Edit View