Application of BRET to monitor ligand binding to GPCRs
Stoddart, Leigh A. and Johnstone, Elizabeth K.M. and Wheal, Amanda J. and Goulding, Joelle and Robers, Matthew B. and Machleidt, Thomas and Wood, Keith V. and Hill, Stephen J. and Pfleger, Kevin D.G. (2015) Application of BRET to monitor ligand binding to GPCRs. Nature Methods, 12 . pp. 661-663. ISSN 1548-7105
Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein–coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.
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