Application of BRET to monitor ligand binding to GPCRs

Stoddart, Leigh A. and Johnstone, Elizabeth K.M. and Wheal, Amanda J. and Goulding, Joelle and Robers, Matthew B. and Machleidt, Thomas and Wood, Keith V. and Hill, Stephen J. and Pfleger, Kevin D.G. (2015) Application of BRET to monitor ligand binding to GPCRs. Nature Methods, 12 . pp. 661-663. ISSN 1548-7105

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Abstract

Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein–coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.

Item Type: Article
Keywords: cell signalling, drug development, optical spectroscopy
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: https://doi.org/10.1038/nmeth.3398
Depositing User: Eprints, Support
Date Deposited: 19 Jul 2016 13:48
Last Modified: 20 Sep 2016 16:27
URI: http://eprints.nottingham.ac.uk/id/eprint/35176

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