Electrospray deposition in vacuum as method to create functionally active protein immobilization on polymeric substrates

Fornari, Enzo and Roberts, Clive J. and Temperton, Robert H. and O’Shea, James N. (2015) Electrospray deposition in vacuum as method to create functionally active protein immobilization on polymeric substrates. Journal of Colloid and Interface Science, 453 . pp. 252-259. ISSN 1095-7103

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We demonstrate in this work the deposition of a large biological molecule (fibronectin) on polymeric substrates in a high vacuum environment using an electrospray deposition system. Fibronectin was deposited and its distribution and structure investigated and retention of function (ability to promote cell adhesion) on return to liquid environment is shown. AFM was used to monitor changes in the morphology of the surface before and after fibronectin deposition, whilst the biological activity of the deposited protein is assessed through a quantitative analysis of the biomolecular adhesion and migration of fibroblast cells to the modified surfaces. For the first time we have demonstrated that using high vacuum electrospray deposition it is possible to deposit large protein molecules on polymeric surfaces whilst maintaining the protein activity. The deposition of biological molecules such as proteins with the retention of their activity onto clean well-controlled surfaces under vacuum condition, offers the possibility for future studies utilizing high resolution vacuum based techniques at the atomic and molecular scale providing a greater understanding of protein–surface interface behaviour of relevance to a wide range of applications such as in sensors, diagnostics and tissue engineering.

Item Type: Article
Keywords: Vacuum electrospray deposition; Fibronectin deposition; Cell adhesion and migration; Protein activity
Schools/Departments: University of Nottingham UK Campus > Faculty of Science > School of Physics and Astronomy
Identification Number: https://doi.org/10.1016/j.jcis.2015.05.007
Depositing User: O'Shea, Dr James N
Date Deposited: 26 Jul 2016 10:58
Last Modified: 24 Sep 2016 16:36
URI: http://eprints.nottingham.ac.uk/id/eprint/34931

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