The impact of surface chemistry modification on macrophage polarisation

Rostam, Hassan M. and Singh, Sonali and Salazar, Fabian and Magennis, Peter and Hook, Andrew L. and Singh, Taranjit and Vrana, Nihal E. and Alexander, Morgan R. and Ghaemmaghami, Amir M. (2016) The impact of surface chemistry modification on macrophage polarisation. Immunobiology . ISSN 1878-3279 (In Press)

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Macrophages are innate immune cells that have a central role in combating infection and maintaining tissue homeostasis. They exhibit remarkable plasticity in response to environmental cues. At either end of a broad activation spectrum are pro-inflammatory (M1) and anti-inflammatory (M2) macrophages with distinct functional and phenotypical characteristics. Macrophages also play a crucial role in orchestrating immune responses to biomaterials used in the fabrication of implantable devices and drug delivery systems. To assess the impact of different surface chemistries on macrophage polarisation, human monocytes were cultured for 6 days on untreated hydrophobic polystyrene (PS) and hydrophilic O2 plasma-etched polystyrene (O2-PS40) surfaces. Our data clearly show that monocytes cultured on the hydrophilic O2-PS40 surface are polarised towards an M1-like phenotype, as evidenced by significantly higher expression of the pro-inflammatory transcription factors STAT1 and IRF5. By comparison, monocytes cultured on the hydrophobic PS surface exhibited an M2-like phenotype with high expression of mannose receptor (MR)and production of the anti-inflammatory cytokines IL-10 and CCL18. While the molecular basis of such different patterns of cell differentiation is yet to be fully elucidated, we hypothesise that it is due to the adsorption of different biomolecules on these surface chemistries. Indeed our surface characterisation data show quantitative and qualitative differences between the protein layers on the O2-PS40 surface compared to PS surface which could be responsible for the observed differential macrophage polarisation on each surface.

Item Type: Article
Keywords: Macrophage; Monocyte; M1; M2; Macrophage polarisation; Biomaterials; Surface chemistry; Oxygen plasma etching; Water contact angle; Foreign body response
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Life Sciences
University of Nottingham UK Campus > Faculty of Science > School of Pharmacy
Identification Number:
Depositing User: Singh, Sonali
Date Deposited: 06 Jul 2016 14:21
Last Modified: 15 Sep 2016 18:47

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