Enzyme characterisation and kinetic modelling of the pentose phosphate pathway in yeast

Messiha, Hanan L. and Kent, Edward and Malys, Naglis and Carroll, Kathleen M. and Swainston, Neil and Mendes, Pedro and Smallbone, Kieran (2014) Enzyme characterisation and kinetic modelling of the pentose phosphate pathway in yeast. PeerJ, 2 (e146v4). ISSN 2167-8359

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We present the quantification and kinetic characterisation of the enzymes of the pentose phosphate pathway in Saccharomyces cerevisiae. The data are combined into a mathematical model that describes the dynamics of this system and allows us to predict changes in metabolite concentrations and fluxes in response to perturbations. We use the model to study the response of yeast to a glucose pulse. We then combine the model with an existing glycolysis model to study the effect of oxidative stress on carbohydrate metabolism. The combination of these two models was made possible by the standardised enzyme kinetic experiments carried out in both studies. This work demonstrates the feasibility of constructing larger network-scale models by merging smaller pathway-scale models.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: https://doi.org/10.7287/peerj.preprints.146v4
Depositing User: Malys, Naglis
Date Deposited: 06 Jul 2016 10:39
Last Modified: 12 Oct 2017 21:00
URI: http://eprints.nottingham.ac.uk/id/eprint/34654

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