Brown, D.M. and Williams, H. and Ryan, K.J.P. and Wilson, T.L. and Daniel, Zoe and Mareko, M.H.D. and Emes, Richard D. and Harris, D.W. and Jones, Sarah and Wattis, Jonathan A.D. and Dryden, Ian L. and Hodgman, Charlie and Brameld, John M. and Parr, Tim
Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) and serine biosynthetic pathway genes are co-ordinately increased during anabolic agent-induced skeletal muscle growth.
Scientific Reports, 6
We aimed to identify novel molecular mechanisms for muscle growth during administration of anabolic agents. Growing pigs (Duroc/(Landrace/Large-White)) were administered Ractopamine (a beta-adrenergic agonist; BA; 20 ppm in feed) or Reporcin (recombinant growth hormone; GH; 10 mg/48 hours injected) and compared to a control cohort (feed only; no injections) over a 27-day time course (1, 3, 7, 13 or 27-days). Longissimus Dorsi muscle gene expression was analyzed using Agilent porcine transcriptome microarrays and clusters of genes displaying similar expression profiles were identified using a modified maSigPro clustering algorithm. Anabolic agents increased carcass (p = 0.002) and muscle weights (Vastus Lateralis: p < 0.001; Semitendinosus: p = 0.075). Skeletal muscle mRNA expression of serine/one-carbon/glycine biosynthesis pathway genes (Phgdh, Psat1 and Psph) and the gluconeogenic enzyme, phosphoenolpyruvate carboxykinase-M (Pck2/PEPCK-M), increased during treatment with BA, and to a lesser extent GH (p < 0.001, treatment x time interaction). Treatment with BA, but not GH, caused a 2-fold increase in phosphoglycerate dehydrogenase (PHGDH) protein expression at days 3 (p < 0.05) and 7 (p < 0.01), and a 2-fold increase in PEPCK-M protein expression at day 7 (p < 0.01). BA treated pigs exhibit a profound increase in expression of PHGDH and PEPCK-M in skeletal muscle, implicating a role for biosynthetic metabolic pathways in muscle growth.
||Ractopamine, Reporcin, Anabolic, Pigs, Metabolism, Muscle
||University of Nottingham UK Campus > Faculty of Science > School of Biosciences > Division of Agricultural and Environmental Sciences
University of Nottingham UK Campus > Faculty of Science > School of Biosciences > Division of Nutritional Sciences
University of Nottingham UK Campus > Faculty of Science > School of Mathematical Sciences
University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
||04 Jul 2016 12:17
||21 Sep 2016 15:02
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