Synthesis of19F nucleic acid–polymer conjugates as real-time MRI probes of biorecognition

Sicilia, Giovanna and Davis, Adrienne L. and Spain, Sebastian G. and Magnusson, Johannes P. and Boase, Nathan R. B. and Thurecht, Kristofer J. and Alexander, Cameron (2016) Synthesis of19F nucleic acid–polymer conjugates as real-time MRI probes of biorecognition. Polymer Chemistry, 7 (12). pp. 2180-2191. ISSN 1759-9962

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Abstract

Polymer-DNA conjugates in which one nucleic acid strand contains fluorine-substituted nucleobases have been prepared and characterised. The efficacy of these novel 19F nucleic acid − polymer conjugates as sensitive and selective in vitro reporters of DNA binding events is demonstrated through a number of rapid-acquisition MR sequences. The conjugates respond readily and in a sequence specific manner to external target oligonucleotide sequences by changes in hybridisation. In turn, these structural changes in polymer-nucleotide conjugates translate into responses which are detectable in fluorine relaxation and diffusion switches, and which can be monitored by in vitro Spin Echo and DOSY NMR spectroscopy. Although complementary to conventional FRET methods, the excellent diagnostic properties of fluorine nuclei make this approach a versatile and sensitive probe of molecular structure and conformation in polymeric assemblies.

Item Type: Article
Schools/Departments: University of Nottingham UK Campus > Faculty of Science > School of Chemistry
University of Nottingham UK Campus > Faculty of Science > School of Pharmacy
Identification Number: https://doi.org/10.1039/c5py01883h
Depositing User: Eprints, Support
Date Deposited: 18 Jul 2016 08:04
Last Modified: 25 Sep 2016 01:53
URI: http://eprints.nottingham.ac.uk/id/eprint/34466

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