Dual signal transduction pathways activated by TSH receptors in rat primary tanycyte cultures

Bolborea, Matei and Helfer, Gisela and Ebling, Francis J.P. and Barrett, Perry (2015) Dual signal transduction pathways activated by TSH receptors in rat primary tanycyte cultures. Journal of Molecular Endocrinology, 54 . pp. 241-250. ISSN 1479-6813

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Tanycytes play multiple roles in hypothalamic functions, including sensing peripheral nutrients and metabolic hormones, regulating neurosecretion and mediating seasonal cycles of reproduction and metabolic physiology. This last function reflects the expression of TSH receptors in tanycytes, which detect photoperiod-regulated changes in TSH secretion from the neighbouring pars tuberalis. The present overall aim was to determine the signal transduction pathway by which TSH signals in tanycytes. Expression of the TSH receptor in tanycytes of 10-day-old Sprague Dawley rats was observed by in situ hybridisation. Primary ependymal cell cultures prepared from 10-day-old rats were found by immunohistochemistry to express vimentin but not GFAP and by PCR to express mRNA for Dio2, Gpr50, Darpp-32 and Tsh receptors that are characteristic of tanycytes. Treatment of primary tanycyte/ependymal cultures with TSH (100 IU/l) increased cAMP as assessed by ELISA and induced a cAMP-independent increase in the phosphorylation of ERK1/2 as assessed by western blot analysis. Furthermore, TSH (100 IU/l) stimulated a 2.17-fold increase in Dio2 mRNA expression. We conclude that TSH signal transduction in cultured tanycytes signals via Gαs to increase cAMP and via an alternative G protein to increase phosphorylation of ERK1/2.

Item Type: Article
Additional Information: Disclaimer: this is not the definitive version of record of this article. This manuscript has been accepted for publication in Journal of Molecular Endocrinology, but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, Bioscientifica accepts no responsibility for any errors or omissions it may contain. The definitive version is now freely available at 10.1530/JME-14-0298 2015.
Keywords: Thyrotrophin, Pars tuberalis, Median eminence, Neuroendocrine
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: https://doi.org/10.1530/JME-14-0298
Depositing User: Eprints, Support
Date Deposited: 23 Jun 2016 10:00
Last Modified: 14 Sep 2016 07:41
URI: http://eprints.nottingham.ac.uk/id/eprint/34340

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