Technical considerations for the generation of novel pseudotyped viruses

King, Barnabas and Temperton, Nigel and Grehan, Keith and Scott, Simon D. and Wright, Edward and Tarr, Alexander W. and Daly, Janet M. (2016) Technical considerations for the generation of novel pseudotyped viruses. Future Virology, 11 (1). pp. 47-59. ISSN 1746-0808

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Abstract

A pseudotyped virus (PV) is a virus particle with an envelope protein originating from a different virus. The ability to dictate which envelope proteins are expressed on the surface has made pseudotyping an important tool for basic virological studies such as determining the cellular targets of the envelope protein as well as identification of potential antiviral compounds and measuring specific antibody responses. In this review, we describe the common methodologies employed to generate pseudotyped viruses (PVs) with a focus on approaches to improve the efficacy of PV generation.

Item Type: Article
Keywords: Pseudotyped virus, pseudotypes, pseudoparticles, envelope glycoproteins
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: https://doi.org/10.2217/fvl.15.106
Depositing User: Daly, Janet
Date Deposited: 21 Jun 2016 09:55
Last Modified: 14 Sep 2016 22:10
URI: http://eprints.nottingham.ac.uk/id/eprint/34242

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