SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance

Asiani, Karishma R. and Williams, Huw Edward Llewelyn and Bird, Louise and Jenner, Matthew and Searle, Mark S. and Hobman, Jon L. and Scott, David J. and Soultanas, Panos (2016) SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance. Molecular Microbiology . ISSN 1365-2958

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Abstract

Ag+ resistance was initially found on the Salmonella enetrica serovar Typhimurium multi-resistance plasmid pMG101 from burns patients in 1975. The putative model of Ag+ resistance, encoded by the sil operon from pMG101, involves export of Ag+ via an ATPase (SilP), an effluxer complex (SilCFBA) and a periplasmic chaperon of Ag+ (SilE). SilE is predicted to be intrinsically disordered. We tested this hypothesis using structural and biophysical studies and show that SilE is an intrinsically disordered protein in its free apo-form but folds to a compact structure upon optimal binding to six Ag+ ions in its holo-form. Sequence analyses and site-directed mutagenesis established the importance of histidine and methionine containing motifs for Ag+-binding, and identified a nucleation core that initiates Ag+-mediated folding of SilE. We conclude that SilE is a molecular sponge for absorbing metal ions.

Item Type: Article
Schools/Departments: University of Nottingham UK Campus > Faculty of Science > School of Biosciences
University of Nottingham UK Campus > Faculty of Science > School of Chemistry
Identification Number: https://doi.org/10.1111/mmi.13399
Related URLs:
URLURL Type
http://www.ncbi.nlm.nih.gov/pubmed/27085056UNSPECIFIED
Depositing User: Soultanas, Prof Panos
Date Deposited: 24 Jun 2016 12:07
Last Modified: 17 Sep 2016 02:35
URI: http://eprints.nottingham.ac.uk/id/eprint/34211

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