Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging

Sinjab, Faris and Kong, Kenny and Gibson, Graham and Varma, Sandeep and Williams, Hywel C. and Padgett, Miles and Notingher, Ioan (2016) Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging. Biomedical Optics Express, 7 (8). pp. 2993-3006. ISSN 2156-7085

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Abstract

We describe a multifocal Raman micro-spectroscopy detection method based on a digital micromirror device, which allows for simultaneous “power-sharing” acquisition of Raman spectra from ad hoc sampling points. As the locations of the points can be rapidly updated in real-time via software control of a liquid-crystal spatial light modulator (LC-SLM), this technique is compatible with automated adaptive- and selective-sampling Raman spectroscopy techniques, the latter of which has previously been demonstrated for fast diagnosis of skin cancer tissue resections. We describe the performance of this instrument and show examples of multiplexed measurements on a range of test samples. Following this, we show the feasibility of reducing measurement time for power-shared multifocal Raman measurements combined with confocal auto-fluorescence imaging to provide guided diagnosis of tumours in human skin samples.

Item Type: Article
Schools/Departments: University of Nottingham UK Campus > Faculty of Science > School of Physics and Astronomy
Identification Number: https://doi.org/10.1364/BOE.7.002993
Depositing User: Eprints, Support
Date Deposited: 18 Jul 2016 07:50
Last Modified: 14 Sep 2016 08:04
URI: http://eprints.nottingham.ac.uk/id/eprint/34189

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