Fructose-1,6-bisphosphate aldolase of Neisseria meningitidis binds human plasminogen via its C-terminal lysine residue

Shams, Fariza and Oldfield, Neil J. and Lai, Si Kei and Tunio, Sarfraz Ali and Wooldridge, Karl G. and Turner, David P.J. (2016) Fructose-1,6-bisphosphate aldolase of Neisseria meningitidis binds human plasminogen via its C-terminal lysine residue. Microbiology Open, 5 (2). pp. 340-350. ISSN 2045-8827

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Abstract

Neisseria meningitidis is a leading cause of fatal sepsis and meningitis worldwide. As for commensal species of human neisseriae, N. meningitidis inhabits the human nasopharynx and asymptomatic colonization is ubiquitous. Only rarely does the organism invade and survive in the bloodstream leading to disease. Moonlighting proteins perform two or more autonomous, often dissimilar, functions using a single polypeptide chain. They have been increasingly reported on the surface of both prokaryotic and eukaryotic organisms and shown to interact with a variety of host ligands. In some organisms moonlighting proteins perform virulence-related functions, and they may play a role in the pathogenesis of N. meningitidis. Fructose-1,6- bisphosphate aldolase (FBA) was previously shown to be surface-exposed in meningococci and involved in adhesion to host cells. In this study, FBA was shown to be present on the surface of both pathogenic and commensal neisseriae, and surface localization and anchoring was demonstrated to be independent of aldolase activity. Importantly, meningococcal FBA was found to bind to human glu- plasminogen in a dose-dependent manner. Site-directed mutagenesis demonstrated that the C-terminal lysine residue of FBA was required for this interaction, whereas # subterminal lysine residues were not involved.

Item Type: Article
Keywords: Aldolase, Neisseria meningitidis, pathogenesis, plasminogen, protein moonlighting.
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Life Sciences > School of Molecular Medical Sciences > Bacteriology
Identification Number: https://doi.org/10.1002/mbo3.331
Depositing User: Turner, David
Date Deposited: 20 Jun 2016 09:57
Last Modified: 20 Sep 2016 03:55
URI: http://eprints.nottingham.ac.uk/id/eprint/34182

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