On the pivotal role of PPARa in adaptation of the heart to hypoxia and why fat in the diet increases hypoxic injury

Cole, Mark and Abd Jamil, Amira H. and Heather, Lisa C. and Murray, Andrew J. and Sutton, Elizabeth R. and Slingo, Mary and Sebag-Montefiore, Liam and Tan, Suat Cheng and Aksentijević, Dunja and Gildea, Ottilie S. and Stuckey, Daniel J. and Yeoh, Kar Kheng and Carr, Carolyn A. and Evans, Rhys D. and Aasum, Ellen and Schofield, Christopher J. and Ratcliffe, Peter J. and Neubauer, Stefan and Robbins, Peter A. and Clarke, Kieran (2016) On the pivotal role of PPARa in adaptation of the heart to hypoxia and why fat in the diet increases hypoxic injury. FASEB Journal, 30 (8). pp. 2684-2697. ISSN 0892-6638

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The role of peroxisome proliferator activated alpha (PPARα) -mediated metabolic remodeling in cardiac adaptation to hypoxia has yet to be defined. Here, mice were housed in hypoxia for 3 weeks before in vivo contractile function was measured using cine magnetic resonance (MR) imaging. In isolated, perfused hearts, energetics were measured using 31P MR spectroscopy and glycolysis and fatty acid oxidation were measured using 3H labelling. Compared with normoxic, chow-fed control mouse heart, hypoxia decreased PPARα expression, fatty acid oxidation and mitochondrial UCP3 levels, while increasing glycolysis, all of which served to maintain normal ATP concentrations and thereby ejection fractions. A high-fat diet increased cardiac PPARα expression, fatty acid oxidation and UCP3 levels, with decreased glycolysis. Hypoxia was unable to alter the high PPARα expression or reverse the metabolic changes caused by the high fat diet, with the result that ATP concentrations and contractile function decreased significantly. The adaptive metabolic changes caused by hypoxia in control mouse hearts were found to have already occurred in PPARα-/- mouse hearts, and sustained function in hypoxia despite an inability for further metabolic remodelling. We conclude that decreased cardiac PPARα expression is essential for adaptive metabolic remodelling in hypoxia, but is prevented by dietary fat.

Item Type: Article
Keywords: Cardiac contractile function; Cine magnetic resonance imaging; Hypoxia inducible factor (HIF); Myocardial energy metabolism; 31 P Magnetic resonance spectroscopy; Substrate metabolism
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Life Sciences > School of Biomedical Sciences
Identification Number: https://doi.org/10.1096/fj.201500094R
Depositing User: Cole, Mark
Date Deposited: 17 Jun 2016 08:30
Last Modified: 14 Sep 2016 08:08
URI: http://eprints.nottingham.ac.uk/id/eprint/34100

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