Imaging early endothelial inflammation following stroke by core shell silica superparamagnetic glyconanoparticles that target selectin
Farr, Tracy D. and Lai, Chian-Hui and Grünstein, Dan and Orts-Gil, Guillermo and Wang, Cheng-Chung and Boehm-Sturm, Philipp and Seeberger, Peter H. and Harms, Christoph (2014) Imaging early endothelial inflammation following stroke by core shell silica superparamagnetic glyconanoparticles that target selectin. Nano Letters, 14 (4). pp. 2130-2134. ISSN 1530-6992
Official URL: http://dx.doi.org/10.1021/nl500388h
Activation of the endothelium is a pivotal first step for leukocyte migration into the diseased brain. Consequently, imaging this activation process is highly desirable. We synthesized carbohydrate-functionalized magnetic nanoparticles that bind specifically to the endothelial transmembrane inflammatory proteins E and P selectin. Magnetic resonance imaging revealed that the targeted nanoparticles accumulated in the brain vasculature following acute administration into a clinically relevant animal model of stroke, though increases in selectin expression were observed in both brain hemispheres. Nonfunctionalized naked particles also appear to be a plausible agent to target the ischemic vasculature. The importance of these findings is discussed regarding the potential for translation into the clinic.
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