Mass spectrometry insights into a tandem ubiquitin-binding domain hybrid engineered for the selective recognition of unanchored polyubiquitin

Scott, Daniel and Garner, Tom P. and Long, Jed and Strachan, Jo and Mistry, Sharad C. and Bottrill, Andrew R. and Tooth, David J. and Searle, Mark S. and Oldham, Neil J. and Layfield, Rob (2016) Mass spectrometry insights into a tandem ubiquitin-binding domain hybrid engineered for the selective recognition of unanchored polyubiquitin. Proteomics, 16 (14). pp. 1961-1969. ISSN 1615-9861

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Abstract

Unanchored polyubiquitin chains are emerging as importanregulators of cellular physiology with diverse roles paralleling those of substrate-conjugated polyubiquitin. However tools able to discriminate unanchored polyubiquitin chains of different isopeptide linkages have not been described. We describe the design of a linker-optimised ubiquitin-binding domain hybrid (t-UBD) containing two UBDs, a ZnF-UBP domain in tandem with a linkage-selective UBA domain, which exploits avidity effects to afford selective recognition of unanchored Lys48-linked polyubiquitin chains. Utilising native MS to quantitatively probe binding affinities we confirm cooperative binding of the UBDs within the synthetic protein, and desired binding specificity for Lys48-linked ubiquitin dimers. Furthermore MS/MS analyses indicate that the t-UBD, when applied as an affinity enrichment reagent, can be used to favour the purification of endogenous unanchored Lys48-linked polyubiquitin chains from mammalian cell extracts. Our study indicates that strategies for the rational design and engineering of polyubiquitin chain-selective binding in non-biological polymers are possible, paving the way for the generation of reagents to probe unanchored polyubiquitin chains of different linkages and more broadly the ‘ubiquitome’.

Item Type: Article
Additional Information: "This is the peer reviewed version of the following article: Scott, Daniel and Garner, Tom P. and Long, Jed and Strachan, Jo and Mistry, Sharad C. and Bottrill, Andrew R. and Tooth, David J. and Searle, Mark S. and Oldham, Neil J. and Layfield, Rob (2016) Mass spectrometry insights into a tandem ubiquitin-binding domain hybrid engineered for the selective recognition of unanchored polyubiquitin. Proteomics. ISSN 1615-9853, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/pmic.201600067/abstract. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: https://doi.org/10.1002/pmic.201600067
Depositing User: Eprints, Support
Date Deposited: 04 Apr 2016 13:12
Last Modified: 14 Sep 2016 09:52
URI: http://eprints.nottingham.ac.uk/id/eprint/32622

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