The limitations of in vitro experimentation in understanding biofilms and chronic infection

Roberts, Aled and Kragh, Kasper and Bjarnsholt, Thomas and Diggle, Stephen P. (2015) The limitations of in vitro experimentation in understanding biofilms and chronic infection. Journal of Molecular Biology, 427 (23). pp. 3646-3661. ISSN 1089-8638

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We have become increasingly aware that during infection, pathogenic bacteria often grow in multi- cellular biofilms which are often highly resistant to antibacterial strategies. In order to understand how biofilms form and contribute to infection, in vitro biofilm systems such as microtitre plate as- says and flow cells, have been heavily used by many research groups around the world. Whilst these methods have greatly increased our understanding of the biology of biofilms, it is becoming increasingly apparent that many of our in vitro methods do not accurately represent in vivo conditions. Here we present a systematic review of the most widely used in vitro biofilm systems, and we discuss why they are not always representative of the in vivo biofilms found in chronic infections. We present examples of methods that will help us to bridge the gap between in vitro and in vivo biofilm work, so that our bench-side data can ultimately be used to improve bedside treatment.

Item Type: Article
Keywords: biofilm; chronic infection
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number:
Depositing User: Diggle, Dr Stephen
Date Deposited: 24 Feb 2016 16:11
Last Modified: 14 Sep 2016 02:46

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