Screening exons 16 and 17 of the amyloid precursor protein gene in sporadic early-onset Alzheimer’s disease

Barber, Imelda S. and García-Cárdenas, Jennyfer M. and Sakdapanichkul, Chidchanok and Deacon, Christopher and Zapata Erazo, Gabriela and Guerreiro, Rita and Bras, Jose and Hernandez, Dena and Singleton, Andrew and Guetta-Baranes, Tamar and Braae, Anne and Clement, Naomi and Patel, Tulsi and Brookes, Keeley and Medway, Christopher and Chappell, Sally and Mann, David M. (2016) Screening exons 16 and 17 of the amyloid precursor protein gene in sporadic early-onset Alzheimer’s disease. Neurobiology of Aging, 39 . 220.e1-220.e7. ISSN 1558-1497 (In Press)

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Early-onset Alzheimer’s disease (EOAD) can be familial (FAD) or sporadic (sEOAD); both have a disease onset ≤ 65 years of age. 451 sEOAD samples were screened for known causative mutations in exon 16 and 17 of the Amyloid Precursor Protein gene (APP). Four samples were shown to be heterozygous for one of three known causative mutations: p.A713T, p.V717I and p.V717G, this highlights the importance of screening EOAD patients for causative mutations. Additionally, we document an intronic 6 base pair (bp) deletion located 83 bp downstream of exon 17 (rs367709245, IVS17 83-88delAAGTAT), which has a nonsignificantly increased minor allele frequency in our sEOAD cohort (0.006) compared to LOAD (0.002) and controls (0.002). To assess the effect of the 6 bp deletion on splicing, COS-7 and BE(2)-C cells were transfected with a minigene vector encompassing exon 17. There was no change in splicing of exon 17 from constructs containing either wild type or deletion inserts. Sequencing of cDNA generated from cerebellum and temporal cortex of a patient harbouring the deletion found no evidence of transcripts with exon 17 removed.

Item Type: Article
Keywords: Alzheimer's disease, early-onset, sporadic, screening, APP, rs367709245
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Life Sciences > School of Molecular Medical Sciences > Human Genetics Research Group
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Depositing User: Barber, Imelda
Date Deposited: 11 Feb 2016 14:46
Last Modified: 14 Sep 2016 20:53

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