Construction of a doxycycline inducible adipogenic lentiviral expression system

Liu, Q. and Hill, P.J. and Karamitri, Angeliki and Ryan, K.J.P. and Chen, H.Y. and Lomax, Michael A. (2013) Construction of a doxycycline inducible adipogenic lentiviral expression system. Plasmid, 69 (1). pp. 96-103. ISSN 0147-619X

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Abstract

To provide a tool for research on regulating adipocyte differentiation, tetracycline inducible (Tet on) lentiviral expression vectors under the control of an adipose-specific promoter were constructed. The lowest basal expression in the absence of doxycycline and most efficient dose-dependent, doxycycline-induced transient overexpression was observed using vectors constructed with a combination of Tetracycline Responsive Element (TRE) and reverse tetracycline-controlled TransActivator advanced (rtTAadv), transfected in white (3T3-L1) and brown (HIB-1B) preadipocytes cell lines. The results demonstrate that doxycycline adipogenic inducible expression can be achieved using a pLenti TRE / rtTA adv under the control of the truncated aP2 promoter in HIB-1B preadipocytes.

Item Type: Article
Keywords: Tetracycline inducible expression; Lentiviral plasmid; Adipose-specific promoter; Adipocyte
Schools/Departments: University of Nottingham UK Campus > Faculty of Science > School of Biosciences
Identification Number: https://doi.org/10.1016/j.plasmid.2012.10.001
Depositing User: Chamberlain, Mr Dick
Date Deposited: 14 May 2014 14:00
Last Modified: 14 Sep 2016 23:15
URI: http://eprints.nottingham.ac.uk/id/eprint/3158

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