Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry

Herod, Morgan R. and Pineda, Robert G. and Mautner, Vivien and Onion, David (2015) Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry. Small, 11 (7). pp. 797-803. ISSN 1613-6829

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Abstract

A quantum dot method for highly efficient labelling of single adenoviral particles is developed. The technique has no impact on viral fitness and allows the imaging and tracking of virus binding and internalisation events using a variety of techniques including imaging cytometry and confocal microscopy. The method is applied to characterise the tropism of different adenoviral vectors.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/744811
Keywords: quantum dots; adenovirus; gene therapy; flow cytometry; imaging cytometry
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine
University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: https://doi.org/10.1002/smll.201401885
Depositing User: Onion, David
Date Deposited: 20 Jan 2016 10:43
Last Modified: 04 May 2020 17:02
URI: http://eprints.nottingham.ac.uk/id/eprint/31291

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