Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System
Blanchard, Adam M. and Leigh, James A. and Egan, Sharon A. and Emes, Richard D. (2015) Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System. Frontiers in Genetics, 6 . e139/1-e139/8. ISSN 1664-8021
The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has beendeveloped for simple conditionally essential genome discovery experiments in bacteria.Capable of using raw sequence data files alongside a FASTA sequence of thereference genome and GFF file, PIMMS will generate a tabulated output of each codingsequence with corresponding mapped insertions accompanied with normalized resultsenabling streamlined analysis. This allows for a quick assay of the genome to identifyconditionally essential genes on a standar d desktop computer prioritizing results forfurther investigation.
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