Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System

Blanchard, Adam M. and Leigh, James A. and Egan, Sharon A. and Emes, Richard D. (2015) Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System. Frontiers in Genetics, 6 . e139/1-e139/8. ISSN 1664-8021

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The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has beendeveloped for simple conditionally essential genome discovery experiments in bacteria.Capable of using raw sequence data files alongside a FASTA sequence of thereference genome and GFF file, PIMMS will generate a tabulated output of each codingsequence with corresponding mapped insertions accompanied with normalized resultsenabling streamlined analysis. This allows for a quick assay of the genome to identifyconditionally essential genes on a standar d desktop computer prioritizing results forfurther investigation.

Item Type: Article
Additional Information: This document is protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission.
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
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Depositing User: Emes, Richard
Date Deposited: 18 Dec 2015 13:51
Last Modified: 12 Oct 2017 20:01

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