Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System

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Blanchard, Adam M., Leigh, James A., Egan, Sharon A. and Emes, Richard D. (2015) Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System. Frontiers in Genetics, 6 . e139/1-e139/8. ISSN 1664-8021

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Abstract

The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has beendeveloped for simple conditionally essential genome discovery experiments in bacteria.Capable of using raw sequence data files alongside a FASTA sequence of thereference genome and GFF file, PIMMS will generate a tabulated output of each codingsequence with corresponding mapped insertions accompanied with normalized resultsenabling streamlined analysis. This allows for a quick assay of the genome to identifyconditionally essential genes on a standar d desktop computer prioritizing results forfurther investigation.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/749943
Additional Information: This document is protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission.
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: 10.3389/fgene.2015.00139
Depositing User: Emes, Richard
Date Deposited: 18 Dec 2015 13:51
Last Modified: 04 May 2020 17:06
URI: https://eprints.nottingham.ac.uk/id/eprint/31135

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