DNA methylation profiling of synovial fluid FLS in rheumatoid arthritis reveals changes common with tissue-derived FLS

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Glossop, John R., Haworth, Kim E., Emes, Richard D., Nixon, Nicola B., Packham, Jon C., Dawes, Peter T., Fryer, Anthony A., Mattey, Derek L. and Farrell, William E. (2015) DNA methylation profiling of synovial fluid FLS in rheumatoid arthritis reveals changes common with tissue-derived FLS. Epigenomics, 7 (4). pp. 539-551. ISSN 1750-1911

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Abstract

Aims: Alterations in DNA methylation contribute to the abnormal phenotype of fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA). Herein, we performed genome-wide DNA methylation profiling of RA FLS derived from synovial fluid, a more readily accessible source of disease-associated cells.

Patients & Methods: Synovial fluid-derived FLS were isolated and expanded in vitro from 12 patients with RA and osteoarthritis (OA). DNA methylation was interrogated using HumanMethylation450 BeadChips and bisulfite pyrosequencing.

Results: Array analysis identified 328 CpGs, representing 195 genes, that were differentially methylated between RA and OA fluid-derived FLS. Comparison with the genes identified in two independent studies of tissue-derived FLS revealed 73 genes in common (~40%), of which 22 shared identity with both studies. Pyrosequencing confirmed altered methylation of these genes.

Conclusions: Synovial fluid-derived RA FLS show methylation changes across multiple genes. A subset of these are common with tissue-derived FLS, supporting the use of fluid-derived FLS for future investigations.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/750513
Keywords: Rheumatoid arthritis; DNA Methylation; Fibroblast-like synoviocyte; Synovial fluid; Epigenetics; Osteoarthritis
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: https://doi.org/10.2217/epi.15.15
Depositing User: Emes, Richard
Date Deposited: 18 Dec 2015 13:19
Last Modified: 04 May 2020 17:07
URI: https://eprints.nottingham.ac.uk/id/eprint/31130

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