Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes

Glossop, John R. and Emes, Richard D. and Nixon, Nicola B. and Packham, Jon C. and Fryer, Anthony A. and Mattey, Derek L. and Farrell, William E. (2016) Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes. Epigenomics, 8 (2). pp. 209-224. ISSN 1750-192X

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (274kB) | Preview

Abstract

AIM: Although aberrant DNA methylation has been described in rheumatoid arthritis (RA), no studies have interrogated this epigenetic modification in early disease. Following recent investigations of T- and B-lymphocytes in established disease, we now characterize in these cell populations genome-wide DNA methylation in treatment-naive patients with early RA.

PATIENTS & METHODS: HumanMethylation450 BeadChips were used to examine genome-wide DNA methylation in lymphocyte populations from 23 early RA patients and 11 healthy individuals.

RESULTS: Approximately 2000 CpGs in each cell type were differentially methylated in early RA. Clustering analysis identified a novel methylation signature in each cell type (150 sites in T-lymphocytes, 113 sites in B-lymphocytes) that clustered all patients separately from controls. A subset of sites differentially methylated in early RA displayed similar changes in established disease.

CONCLUSION: Treatment-naive early RA patients display novel disease-specific DNA methylation aberrations, supporting a potential role for these changes in the development of RA.

Item Type: Article
Keywords: Early rheumatoid arthritis; Treatment-naïve; DNA methylation; T-lymphocyte; B-lymphocyte; CpG; Genome-wide; Illumina 450K array.
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: https://doi.org/10.2217/epi.15.103
Depositing User: Emes, Richard
Date Deposited: 18 Dec 2015 13:09
Last Modified: 17 Nov 2016 19:59
URI: http://eprints.nottingham.ac.uk/id/eprint/30944

Actions (Archive Staff Only)

Edit View Edit View