Fast, ultrasensitive detection of reactive oxygen species using a carbon nanotube based-electrocatalytic intracellular sensorTools Rawson, Frankie J., Hicks, Jacqueline, Dodd, Nicholas, Abate, Wondwossen, Garrett, David J., Yip, Nga-Chi, Fejer, Gyorgy, Downard, Alison J., Baronian, Kim H.R., Jackson, Simon K. and Mendes, Paula M. (2015) Fast, ultrasensitive detection of reactive oxygen species using a carbon nanotube based-electrocatalytic intracellular sensor. ACS Applied Materials and Interfaces, 7 (42). pp. 23527-23537. ISSN 1944-8252 Full text not available from this repository.
Official URL: http://pubs.acs.org/doi/abs/10.1021/acsami.5b06493
AbstractHerein, we report a highly sensitive electrocatalytic sensor-cell construct that can electrochemically communicate with the internal environment of immune cells (e.g., macrophages) via the selective monitoring of a particular reactive oxygen species (ROS), hydrogen peroxide. The sensor, which is based on vertically aligned single-walled carbon nanotubes functionalized with an osmium electrocatalyst, enabled the unprecedented detection of a local intracellular “pulse” of ROS on a short second time scale in response to bacterial endotoxin (lipopolysaccharide-LPS) stimulation. Our studies have shown that this initial pulse of ROS is dependent on NADPH oxidase (NOX) and toll like receptor 4 (TLR4). The results suggest that bacteria can induce a rapid intracellular pulse of ROS in macrophages that initiates the classical innate immune response of these cells to infection.
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