Troubleshooting methods for the generation of novel pseudotyped viruses
King, Barnabas and Temperton, Nigel and Grehan, Keith and Scott, Simon and Wright, Edward and Tarr, Alexander W. and Daly, Janet M. (2016) Troubleshooting methods for the generation of novel pseudotyped viruses. Future Virology . pp. 1-35. ISSN 1746-0808 (In Press)
A pseudotyped virus (PV) is a virus particle with an envelope protein originating from a different virus. The ability to dictate which envelope proteins are expressed on the surface has made pseudotyping an important tool for basic virological studies such as determining the cellular targets of the envelope protein of the virus as well as identification of potential antiviral compounds and measuring specific antibody responses. In this review, we describe the common methodologies employed to generate PVs, with a focus on approaches to improve the efficacy of PV generation.
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