The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology

Rees, Helen C. and Maddison, Ben C. and Middleditch, David J. and Patmore, James R. and Gough, Kevin C. (2014) The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology. Journal of Applied Ecology, 51 . pp. 1450-1459. ISSN 0021-8901

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (233kB) | Preview

Abstract

1. Knowledge of species distribution is critical to ecological management and conservation biology. Effective management requires the detection of populations, which can sometimes be at low densities and is usually based on visual detection and counting.

2. Recently, there has been considerable interest in the detection of short species-specific environmental DNA (eDNA) fragments to allow aquatic species monitoring within different environments due to the potential of greater sensitivity over traditional survey methods which can be time-consuming and costly.

3. Environmental DNA analysis is increasingly being used in the detection of rare or invasive species and has also been applied to eDNA persistence studies and estimations of species biomass and distribution. When combined with next-generation sequencing methods, it has been demonstrated that entire faunas can be identified.

4. Different environments require different sampling methodologies, but there remain areas where laboratory methodologies could be standardized to allow results to be compared across studies.

5. Synthesis and applications. We review recently published studies that use eDNA to moni- tor aquatic populations, discuss the methodologies used and the application of eDNA analysis as a survey tool in ecology. We include innovative ideas for how eDNA can be used for conservation and management citing test cases, for instance, the potential for on-site analyses, including the application of eDNA analysis to carbon nanotube platforms or laser transmission spectroscopy to facilitate rapid on-site detections. The use of eDNA monitoring is already being adopted in the UK for ecological surveys.

Item Type: Article
Additional Information: This is the pre-peer reviewed version of the following article: Rees, H.C., Maddison, B.C., Middleditch, D.J., Patmore, J.R.M., Gough, K.C. (2014), REVIEW: The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology. Journal of Applied Ecology, 51: 1450–1459 doi: 10.1111/1365-2664.12306, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/1365-2664.12306/abstract. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Keywords: DNA Barcoding, Ecosystem Management, eDna, Invasive Species, Next-Generation Sequencing, PCR, Rare or Threatened Species, Species-Specific Detection, Water Sampling
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: https://doi.org/10.1111/1365-2664.12306
Depositing User: Gough, Kevin
Date Deposited: 24 Sep 2015 13:21
Last Modified: 13 Sep 2016 23:39
URI: http://eprints.nottingham.ac.uk/id/eprint/30254

Actions (Archive Staff Only)

Edit View Edit View