A DEMETER-like DNA demethylase protein governs tomato fruit ripening

Liu, Rui E., How-Kit, Alexandre, Stammitti, Linda, Teyssier, Emeline, Rolin, Dominique, Bertrand, Anne, Halle, Stefanie, Liu, Mingchun, Kong, Junhua, Wu, Chaoqun, Degraeve-Guibault, Charlotte, Chapman, Natalie, Maucourt, Mickael, Hodgman, Charlie, Tost, Jörg, Bouzayen, Mondher, Hong, Yiguo, Seymour, Graham B., Giovannoni, James and Gallusci, Philippe (2015) A DEMETER-like DNA demethylase protein governs tomato fruit ripening. Proceedings of the National Academy of Sciences, 112 (34). pp. 10804-10809. ISSN 1091-6490

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In plants, genomic DNA methylation which contributes to development and stress responses can be actively removed by DEMETER-like DNA demethylases (DML). Indeed, in Arabidopsis DMLs are important for maternal imprinting and endosperm demethylation, but only few studies demonstrate the developmental roles of active DNA demethylation conclusively in this plant. Here we show a direct cause and effect relationship between active DNA demethylation mainly mediated by the tomato DML, SlDML2, and fruit ripening; an important developmental process unique to plants. RNAi SlDML2 knock-down results in ripening inhibition via hypermethylation and repression of the expression of genes encoding ripening transcription factors and rate-limiting enzymes of key biochemical processes such as carotenoid synthesis. Our data demonstrate that active DNA demethylation is central to the control of ripening in tomato

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/982781
Keywords: DNA methylation, fruit ripening, tomato, Demeter like
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences > Division of Plant and Crop Sciences
Identification Number: https://doi.org/10.1073/pnas.1503362112
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Depositing User: Seymour, Professor Graham
Date Deposited: 10 Sep 2015 12:19
Last Modified: 04 May 2020 20:07
URI: https://eprints.nottingham.ac.uk/id/eprint/29891

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