Anthelmintic action of plant cysteine proteinases against the rodent stomach nematode, Protospirura muricola, in vitro and in vivo

Stepek, Gillian and Lowe, Ann and Buttle, David J. and Duce, I.R. and Behnke, Jerzy M. (2007) Anthelmintic action of plant cysteine proteinases against the rodent stomach nematode, Protospirura muricola, in vitro and in vivo. Parasitology, 134 (1). pp. 103-112. ISSN 0031-1820

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (566kB) | Preview

Abstract

Cysteine proteinases from the fruit and latex of plants, including papaya, pineapple and fig, were previously shown to have a rapid detrimental effect, in vitro, against the rodent gastrointestinal nematodes, Heligmosomoides polygyrus (which is found in the anterior small intestine) and Trichuris muris (which resides in the caecum). Proteinases in the crude latex of papaya also showed anthelmintic efficacy against both nematodes in vivo. In this paper, we describe the in vitro and in vivo effects of these plant extracts against the rodent nematode, Protospirura muricola, which is found in the stomach. As in earlier work, all the plant cysteine proteinases examined, with the exception of actinidain from the juice of kiwi fruit, caused rapid loss of motility and digestion of the cuticle, leading to death of the nematode in vitro. In vivo, in contrast to the efficacy against H. polygyrus and T. muris, papaya latex only showed efficacy against P. muricola adult female worms when the stomach acidity had been neutralized prior to administration of papaya latex. Therefore, collectively, our studies have demonstrated that, with the appropriate formulation, plant cysteine proteinases have efficacy against nematodes residing throughout the rodent gastrointestinal tract.

Item Type: Article
Additional Information: Copyright Cambridge University Press.
Keywords: Protospirura muricola, plant cysteine proteinases, papaya, pineapple, fig, in vitro, in vivo, antacid, anthelmintic, gastrointestinal nematodes INTRODUCTION
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: https://doi.org/10.1017/S0031182006001302
Depositing User: Behnke, Professor Jerzy M.
Date Deposited: 27 Jul 2015 07:16
Last Modified: 17 Sep 2016 21:05
URI: http://eprints.nottingham.ac.uk/id/eprint/29413

Actions (Archive Staff Only)

Edit View Edit View