Genetic studies on the edible mushrooms Lentinula edodes and Pleurotus species
Fox, Hilary M. (1991) Genetic studies on the edible mushrooms Lentinula edodes and Pleurotus species. PhD thesis, University of Nottingham.
Classical and molecular genetic techniques were investigated as approaches to strain improvement in the edible Basidiomycete species Lentinula (syn. Lenitnus) edodes (Berk.) Pegler (the Shiitake mushroom) and Pleurotus spp. (oyster mushrooms). Central to the theme of breeding these species was the application of protoplasts. Attempts were made to develop a high efficiency isolation and regeneration system for both L. edodes and P. sajor-caju. Relatively low yields of protoplasts from L. edodes and their poor regenerative ability limited their use. Howewer, Pleurotus spp. were found to be quite amenable to protoplast isolation, yielding constantly high yields of viable protoplasts.
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