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Delmas, Stéphane, Duggin, Iain J. and Allers, Thorsten (2013) DNA damage induces nucleoid compaction via the Mre11-Rad50 complex in the archaeon Haloferax volcanii. Molecular Microbiology, 87 (1). pp. 168-179. ISSN 0950-382X

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Abstract

In prokaryotes the genome is organized in a dynamic

structure called the nucleoid, which is embedded in

the cytoplasm. We show here that in the archaeon

Haloferax volcanii, compaction and reorganization of

the nucleoid is induced by stresses that damage the

genome or interfere with its replication. The fraction

of cells exhibiting nucleoid compaction was proportional

to the dose of the DNA damaging agent, and

results obtained in cells defective for nucleotide excision

repair suggest that breakage of DNA strands

triggers reorganization of the nucleoid. We observed

that compaction depends on the Mre11-Rad50

complex, suggesting a link to DNA double-strand

break repair. However, compaction was observed in a

radA mutant, indicating that the role of Mre11-Rad50

in nucleoid reorganisation is independent of homologous

recombination. We therefore propose that

nucleoid compaction is part of a DNA damage

response that accelerates cell recovery by helping

DNA repair proteins to locate their targets, and facilitating

the search for intact DNA sequences during

homologous recombination.

Item Type:	Article
RIS ID:	https://nottingham-repository.worktribe.com/output/1002972
Schools/Departments:	University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences > School of Biology
Identification Number:	https://doi.org/10.1111/mmi.12091
Depositing User:	de Sousa, Mrs Shona
Date Deposited:	01 Apr 2014 10:51
Last Modified:	04 May 2020 20:19
URI:	https://eprints.nottingham.ac.uk/id/eprint/2792

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