Calpain system protein expression in carcinomas of the pancreas, bile duct and ampulla

Storr, Sarah J. and Zaitoun, Abed M. and Arora, Arvind and Durrant, Lindy G. and Lobo, Dileep N. and Madhusudan, Srinivasan and Martin, Stewart G. (2012) Calpain system protein expression in carcinomas of the pancreas, bile duct and ampulla. BMC Cancer, 12 (511). ISSN 1471-2407

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Abstract

Background: Pancreatic cancer, including cancer of the ampulla of Vater and bile duct, is very aggressive and has a

poor five year survival rate; improved methods of patient stratification are required.

Methods: We assessed the expression of calpain-1, calpain-2 and calpastatin in two patient cohorts using

immunohistochemistry on tissue microarrays. The first cohort was composed of 68 pancreatic adenocarcinomas

and the second cohort was composed of 120 cancers of the bile duct and ampulla.

Results: In bile duct and ampullary carcinomas an association was observed between cytoplasmic calpastatin

expression and patient age (P = 0.036), and between nuclear calpastatin expression and increased tumour stage

(P = 0.026) and the presence of vascular invasion (P = 0.043). In pancreatic cancer, high calpain-2 expression was

significantly associated with improved overall survival (P = 0.036), which remained significant in multivariate

Cox-regression analysis (hazard ratio = 0.342; 95% confidence interva l = 0.157-0.741; P = 0.007). In cancers of the

bile duct and ampulla, low cytoplasmic expression of calpastatin was significantly associated with poor overall

survival (P = 0.012), which remained significant in multivariate Cox-regression analysis (hazard ratio = 0.595; 95%

confidence interval = 0.365-0.968; P = 0.037).

Conclusion: The results suggest that calpain-2 and calpastatin expression is important in pancreatic cancers,

influencing disease progression. The findings of this study warrant a larger follow-up study.

Keywords: Calpain, Calpastatin, Pancreas, Ampulla, Bile duct, Cancer

Item Type: Article
Additional Information: Copy of Creative Commons Attribution License must accompany any deposit.
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Life Sciences > School of Molecular Medical Sciences
Depositing User: de Sousa, Mrs Shona
Date Deposited: 01 Apr 2014 10:07
Last Modified: 14 Sep 2016 17:45
URI: http://eprints.nottingham.ac.uk/id/eprint/2784

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