Genetic manipulation of self-incompatibility in diploid potato species
Dzidzienyo, Daniel Kwadjo (2013) Genetic manipulation of self-incompatibility in diploid potato species. PhD thesis, University of Nottingham.
Many of the wild and some cultivated species of potato are true diploids and are therefore more amenable for genetic studies than the majority of tetraploid cultivars. However, the use of these diploid Solanum species is complicated by almost universal self-incompatibility (SI). In Solanum, SI is gametophytic and pistil specificity is controlled by a polymorphic ribonuclease (S-RNase), as found in other members of the Solanaceae. The genetic engineering of self-compatible (SC) diploid potato lines would benefit potato breeding in general and allow inbred lines to be established for the first time. This would facilitate genetic analyses including that of complex traits such as drought resistance or yield. The aim of this thesis is to down regulate the expression of S-RNases in diploid potatoes using the RNAi technique and established procedures for Agrobacterium-mediated transformation. This approach to engineering self-compatibility has already been successfully demonstrated in SI Petunia inflata (Lee et al., 1994) and other species of the Solanaceae.
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