Insights of biosurfactant producing Serratia marcescens strain W2.3 isolated from diseased tilapia fish: a draft genome analysis

Chan, Xin Yue and Chang, Chien Yi and Hong, Kar Wai and Tee, Kok Keng and Yin, Wai Fong and Chan, Kok Gan (2013) Insights of biosurfactant producing Serratia marcescens strain W2.3 isolated from diseased tilapia fish: a draft genome analysis. Gut Pathogens, 5 (Octobe). 5/1-5/5. ISSN 1757-4749

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Abstract

Background

Serratia marcescens is an opportunistic bacterial pathogen with broad range of host ranging from vertebrates, invertebrates and plants. S. marcescens strain W2.3 was isolated from a diseased tilapia fish and it was suspected to be the causal agent for the fish disease as virulence genes were found within its genome. In this study, for the first time, the genome sequences of S. marcescens strain W2.3 were sequenced using the Illumina MiSeq platform.

Result

Several virulent factors of S. marcescens such as serrawettin, a biosurfactant, has been reported to be regulated by N-acyl homoserine lactone (AHL)-based quorum sensing (QS). In our previous studies, an unusual AHL with long acyl side chain was detected from this isolate suggesting the possibility of novel virulence factors regulation. This evokes our interest in the genome of this bacterial strain and hereby we present the draft genome of S. marcescens W2.3, which carries the serrawettin production gene, swrA and the AHL-based QS transcriptional regulator gene, luxR which is an orphan luxR.

Conclusion

With the availability of the whole genome sequences of S. marcescens W2.3, this will pave the way for the study of the QS-mediated genes expression in this bacterium.

Item Type: Article
Keywords: Serratia marcescens, Biosurfactant, Serrawettin, Quorum sensing, N-acyl homoserine lactone, swrA, Next generation sequencing technology
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Life Sciences > School of Molecular Medical Sciences
Identification Number: https://doi.org/10.1186/1757-4749-5-29
Depositing User: Wahid, Ms. Haleema
Date Deposited: 03 Apr 2014 12:18
Last Modified: 13 Sep 2016 16:32
URI: http://eprints.nottingham.ac.uk/id/eprint/2751

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