5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development

Almeida, Rimple D., Loose, Matthew, Sottile, Virginie, Matsa, Elena, Denning, Chris, Young, Lorraine, Johnson, Andrew D., Gering, Martin and Ruzov, Alexey (2012) 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development. Epigenetics, 7 (4). pp. 383-389. ISSN 1559-2294

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Abstract

5-hydroxymethyl-cytosine (5-hmc) is a cytosine modification that is relatively abundant in mammalian pre-implantation

embryos and embryonic stem cells (Esc) derived from mammalian blastocysts. Recent observations imply that both

5-hmc and Tet1/2/3 proteins, catalyzing the conversion of 5-methyl-cytosine to 5-hmc, may play an important role in

self renewal and differentiation of Escs. here we assessed the distribution of 5-hmc in zebrafish and chick embryos

and found that, unlike in mammals, 5-hmc is immunochemically undetectable in these systems before the onset of

organogenesis. In addition, Tet1/2/3 transcripts are either low or undetectable at corresponding stages of zebrafish

development. however, 5-hmc is enriched in later zebrafish and chick embryos and exhibits tissue-specific distribution in adult zebrafish. Our findings show that 5-hmc enrichment of non-committed cells is not a universal feature of vertebrate development and give insights both into evolution of embryonic pluripotency and the potential role of 5-hmc in its regulation.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/1007583
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences
Identification Number: https://doi.org/10.4161/epi.19375
Depositing User: Johnson, Mrs Alison
Date Deposited: 27 Mar 2014 11:33
Last Modified: 04 May 2020 20:21
URI: https://eprints.nottingham.ac.uk/id/eprint/2698

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