5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development

Almeida, Rimple D. and Loose, Matthew and Sottile, Virginie and Matsa, Elena and Denning, Chris and Young, Lorraine and Johnson, Andrew D. and Gering, Martin and Ruzov, Alexey (2012) 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development. Epigenetics, 7 (4). pp. 383-389. ISSN 1559-2294

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Abstract

5-hydroxymethyl-cytosine (5-hmc) is a cytosine modification that is relatively abundant in mammalian pre-implantation

embryos and embryonic stem cells (Esc) derived from mammalian blastocysts. Recent observations imply that both

5-hmc and Tet1/2/3 proteins, catalyzing the conversion of 5-methyl-cytosine to 5-hmc, may play an important role in

self renewal and differentiation of Escs. here we assessed the distribution of 5-hmc in zebrafish and chick embryos

and found that, unlike in mammals, 5-hmc is immunochemically undetectable in these systems before the onset of

organogenesis. In addition, Tet1/2/3 transcripts are either low or undetectable at corresponding stages of zebrafish

development. however, 5-hmc is enriched in later zebrafish and chick embryos and exhibits tissue-specific distribution in adult zebrafish. Our findings show that 5-hmc enrichment of non-committed cells is not a universal feature of vertebrate development and give insights both into evolution of embryonic pluripotency and the potential role of 5-hmc in its regulation.

Item Type: Article
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences
Depositing User: Johnson, Mrs Alison
Date Deposited: 27 Mar 2014 11:33
Last Modified: 15 Sep 2016 08:09
URI: http://eprints.nottingham.ac.uk/id/eprint/2698

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