Allosteric interactions at adenosine A1 and A3 receptors: new insights into the role of small molecules and receptor dimerization

Hill, Stephen J. and May, Lauren T. and Kellam, Barrie and Woolard, Jeanette (2014) Allosteric interactions at adenosine A1 and A3 receptors: new insights into the role of small molecules and receptor dimerization. British Journal of Pharmacology, 171 (5). pp. 1102-1113. ISSN 0007-1188

[img] PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Available under Licence Creative Commons Attribution.
Download (1MB)


Keywords:adenosine;allosterism;receptor;GPCR;dimerization;biased signalling

The purine nucleoside adenosine is present in all cells in tightly regulated concentrations. It is released under a variety of physiological and pathophysiological conditions to facilitate protection and regeneration of tissues. Adenosine acts via specific GPCRs to either stimulate cyclic AMP formation, as exemplified by Gs-protein-coupled adenosine receptors (A2A and A2B), or inhibit AC activity, in the case of Gi/o-coupled adenosine receptors (A1 and A3). Recent advances in our understanding of GPCR structure have provided insights into the conformational changes that occur during receptor activation following binding of agonists to orthosteric (i.e. at the same binding site as an endogenous modulator) and allosteric regulators to allosteric sites (i.e. at a site that is topographically distinct from the endogenous modulator). Binding of drugs to allosteric sites may lead to changes in affinity or efficacy, and affords considerable potential for increased selectivity in new drug development. Herein, we provide an overview of the properties of selective allosteric regulators of the adenosine A1 and A3 receptors, focusing on the impact of receptor dimerization, mechanistic approaches to single-cell ligand-binding kinetics and the effects of A1- and A3-receptor allosteric modulators on in vivo pharmacology.

Item Type: Article
Keywords: adenosine ; allosterism ; receptor ; GPCR ; dimerization ; biased signalling
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences
Identification Number: 10.1111/bph.12345
Depositing User: de Sousa, Mrs Shona
Date Deposited: 26 Mar 2014 11:40
Last Modified: 15 Oct 2017 04:50

Actions (Archive Staff Only)

Edit View Edit View