Botanical extracts from rosehip (rosa canina), willow bark (salix alba), and nettle leaf (urtica dioica) suppress IL-1β-induced NF-κB activation in canine articular chondrocytes

Shakibaei, Mehdi and Allaway, David and Nebrich, Simone and Mobasheri, Ali (2012) Botanical extracts from rosehip (rosa canina), willow bark (salix alba), and nettle leaf (urtica dioica) suppress IL-1β-induced NF-κB activation in canine articular chondrocytes. Evidence-Based Complementary and Alternative Medicine, 2012 . 16/1-16/16. ISSN 1741-427X

[img] PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Available under Licence Creative Commons Attribution.
Download (1MB)

Abstract

The aim of this study was to characterize the anti-inflammatory mode of action of botanical extracts from rosehip (Rosa canina), willow bark (Salix alba), and nettle leaf (Urtica dioica) in an in vitro model of primary canine articular chondrocytes. Methods. The biological effects of the botanical extracts were studied in chondrocytes treated with IL-1β for up to 72 h. Expression of collagen type II, cartilage-specific proteoglycan (CSPG), β1-integrin, SOX-9, COX-2, and MMP-9 and MMP-13 was examined by western blotting. Results. The botanical extracts suppressed IL-1β-induced NF-κB activation by inhibition of IκBα phosphorylation, IκBα degradation, p65 phosphorylation, and p65 nuclear translocation. These events correlated with downregulation of NF-κB targets including COX-2 and MMPs. The extracts also reversed the IL-1β-induced downregulation of collagen type II, CSPG, β1-integrin, and cartilage-specific transcription factor SOX-9 protein expression. In high-density cultures botanical extracts stimulated new cartilage formation even in the presence of IL-1β. Conclusions. Botanical extracts exerted anti-inflammatory and anabolic effects on chondrocytes. The observed reduction of IL-1β-induced NF-κB activation suggests that further studies are warranted to demonstrate the effectiveness of plant extracts in the treatment of OA and other conditions in which NF-κB plays pathophysiological roles.

Item Type: Article
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Depositing User: Johnson, Mrs Alison
Date Deposited: 11 Apr 2014 09:55
Last Modified: 14 Sep 2016 16:26
URI: http://eprints.nottingham.ac.uk/id/eprint/2513

Actions (Archive Staff Only)

Edit View Edit View